Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins
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Effects of cellular ATP depletion on glucose transport and insulin signaling in 3T3-L1 adipocytes.
Glucosamine induced insulin resistance in 3T3-L1 adipocytes, which was associated with a 15% decrease in cellular ATP content. To study the role of ATP depletion in insulin resistance, we employed sodium azide (NaN3) and dinitrophenol (DNP), which affect mitochondrial oxidative phosphorylation, to achieve a similar 15% ATP depletion. Unlike glucosamine, NaN3 and DNP markedly increased basal glu...
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Rapid sequence measures of changes in the rate of 14CO2 production from [14C]glucose bathing the cells was abruptly reduced from 20 to 4 microunits/ml. Interpretation of the data in terms of glucose transport was based on calibration experiments that described the time course of change in 14CO2 production when [14C]glucose entry into adipocytes was slowed by reducing the specific activity of [...
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It is widely accepted that the insulin receptor contains two distinct glycoprotein subunits, a and /?, held together by interchain disulphide bonds and strong non-covalent interactions to yield a (/?-S-S-a)-S-S-(a-S-S-/?) receptor complex (Massague et al., 1980). D-Glucose transporters exist in intracellular storage sites. Stimulation of cells with insulin results in the transporters being sequ...
متن کاملCharacterization of the lag phase of insulin action on glucose transport in rat isolated adipocytes.
Insulin action on glucose transport in isolated rat adipocytes was assessed using the method originally reported by Whitesell et al. Insulin increased the Vmax of glucose transport without affecting the Km. So far as concentration dependence of insulin action it had pH and temperature dependency, and analogue specificity quite similar to previous reports. The lag phase (time necessary for full ...
متن کاملRegulation of insulin-stimulated glucose transport by chronic glucose exposure in 3T3-L1 adipocytes.
Chronic hyperglycemia causes insulin resistance, termed glucose toxicity. Herein we studied chronic glucose-dependent regulation of the glucose transport system in adipocytes. 3T3-L1 adipocytes were incubated for up to 24 h with low (1 mM) or high (25 mM) glucose, and glucose transport was subsequently analyzed. 100 nM insulin was present throughout the experiments. 24 h incubation with 1 mM gl...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Endocrinology and Metabolism
سال: 2007
ISSN: 0193-1849,1522-1555
DOI: 10.1152/ajpendo.00284.2006